Immunofluorescent analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody (Product # MA3-935) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA3-935 detects OATP8 from human tissue and cells. This antibody does not detect dog, mouse or rat protein. MA3-935 also detects the OATP2 isoform.
MA3-935 has been successfully used in immunohistochemistry, immunofluorescence and immunoprecipitation procedures. Immunofluorescence staining of OATP8 in human liver samples results in a pattern consistent with plasma membrane staining.
The MA3-935 immunogen is a synthetic peptide corresponding ot residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.
The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma.
MA3-935 was used in immunohistochemistry, immunoprecipitation and western blot to detect organic anion transporters SLC21A6 and SLC21A8 in human liver and hepatocellular carcinoma
|Cui Y,König J,Nies AT,Pfannschmidt M,Hergt M,Franke WW,Alt W,Moll R,Keppler D||Laboratory investigation; a journal of technical methods and pathology (83:527)||2003|