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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human Oct-2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, mouse, porcine, rabbit based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
Oct-2 is a transcription factor belonging to the POU homeo-domain family that binds to the Ig gene octomer sites regulating B cell specific genes. Oct-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. Oct-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
homeobox protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; OCT2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; POU domain, class 2, transcription factor 2
Oct-2; OCT2; OTF2; POU2F2