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Western blot analysis was performed on whole cell extracts (30 ug lysate) of Raji (Lane 1), Ramos (Lane 2), U-87MG (Lane 3) and Human PBMC (Lane 4). The blots were probed with Anti-OCT2 Mouse Monoclonal Antibody (Product# 395400, 1-2 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A ~ 62 kDa band corresponding to OCT2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from the C-terminus of human Oct2.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OCT-2 is a transcription factor belonging to the POU homeo-domain family that binds to the Ig gene octomer sites regulating B cell specific genes. OCT-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. OCT-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
class 2; homeobox protein; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; OCT2; octamer-binding protein 2; octamer-binding transcription factor 2; OTF-2; OTF2; POU domain; transcription factor 2
Oct-2; OCT2; OTF2; POU2F2