|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein specific to the amino terminus of human Oct-4A|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:600|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction.
A13998 was used in immunocytochemistry to analyze cloned mouse embryos mophokinetics after treatment with epigenetic drugs and blastocyst prediction
|Mallol A,Piqué L,Santaló J,Ibáñez E||Reproduction (Cambridge, England) (151:203)||2016|
Endosialin¿expressing bone sarcoma stem¿like cells are highly tumor¿initiating and invasive.
A13998 was used in immunocytochemistry and western blot to assess endosialin-expressing bone sarcoma stem-like cells and their invasive and highly-tumor initiating properties
|Sun DX,Liao GJ,Liu KG,Jian H||Molecular medicine reports (12:5665)||2015|
Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration.
A13998 was used in immunocytochemistry to research human iPS-derived motor neurons from sporadic ALS patients for gene expression and a strong association between neurodegeneration and mitochondrial functions
|Alves CJ,Dariolli R,Jorge FM,Monteiro MR,Maximino JR,Martins RS,Strauss BE,Krieger JE,Callegaro D,Chadi G||Frontiers in cellular neuroscience (9:null)||2015|
|Human||Not Cited||Cellular characterization of human pluripotent stem cells.||Quintanilla RH||Methods in molecular biology (Clifton, N.J.) (997:179)||2013|