|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human OLIG2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA5-15810 targets OLIG2 in IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15810 immunogen is purified recombinant fragment of human OLIG2 expressed in E. Coli.
MA5-15810 detects OLIG2 which has a predicted molecular weight of approximately 32kDa.
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex.
MA5-15810 was used in immunocytochemistry to study Pax6 expression in murine basal progenitors
|Wong FK,Fei JF,Mora-Bermúdez F,Taverna E,Haffner C,Fu J,Anastassiadis K,Stewart AF,Huttner WB||PLoS biology (13:null)||2015|