|Tested species reactivity||Bovine, Hamster, Human, Mouse, Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
In Western blot, multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.
OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.
MA5-16149 was used in western blot to determine how mitochondrial homeostasis is affected by ubiquitin-mediated regulation of E3 ligase GP78 by MGRN1 in trans
|Mukherjee R,Chakrabarti O||Journal of cell science (129:757)||2016|
Mechanical ventilation triggers abnormal mitochondrial dynamics and morphology in the diaphragm.
MA5-16149 was used in western blot to study the effect of diaphragm contractile inactivity on mitochondrial function.
|Picard M,Azuelos I,Jung B,Giordano C,Matecki S,Hussain S,White K,Li T,Liang F,Benedetti A,Gentil BJ,Burelle Y,Petrof BJ||Journal of applied physiology (Bethesda, Md. : 1985) (118:1161)||2015|