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Invitrogen
OPA1 Monoclonal Antibody (1E8-1D9)
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FIGURE: 1 / 9
OPA1 Antibody (MA5-16149) in ICC/IF
Immunofluorescence analysis of OPA1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with OPA1 Monoclonal Antibody (Product # MA5-16149) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1E8-1D9
Immunogen
Human OPA1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot, multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.
Target Information
OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dynamin-like 120 kDa protein, mitochondrial; dynamin-like guanosine triphosphatase; GTPase; large GTP-binding protein; large GTPase; OPA1; largeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; optic atrophy protein 1; optic atrophy protein 1 homolog; RN protein; unnamed protein product
Gene Aliases: 1200011N24Rik; BERHS; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; MTDPS14A; MTDPS14B; NPG; NTG
UniProt ID: (Human) O60313, (Mouse) P58281, (Rat) Q2TA68
Entrez Gene ID: (Bovine) 524142, (Human) 4976, (Mouse) 74143, (Rat) 171116, (Chinese hamster) 100751873
mitochondrial fission
mitochondrion organization
inner mitochondrial membrane organization
visual perception
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
axonal transport of mitochondrion
positive regulation of interleukin-17 production
cristae formation
negative regulation of apoptotic process
GTP metabolic process
protein oligomerization
membrane fusion
negative regulation of release of cytochrome c from mitochondria
cellular senescence
membrane tubulation
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
mitochondrial inner membrane fusion
positive regulation of T-helper 17 cell lineage commitment
negative regulation of intrinsic apoptotic signaling pathway
neural tube closure
positive regulation of mitochondrial fusion
positive regulation of neuron maturation
calcium ion transmembrane import into mitochondrion
positive regulation of insulin receptor signaling pathway
intracellular distribution of mitochondria
positive regulation of dendritic spine morphogenesis
positive regulation of dendrite development
positive regulation of cellular response to insulin stimulus
response to muscle activity
response to nutrient levels
response to electrical stimulus
retina development in camera-type eye
cellular response to glucose stimulus
cellular response to hypoxia
cochlea development
response to curcumin
cellular response to L-glutamate
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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