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Western Blot. Extracts of rat brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with the µ-opioid receptor antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and signals were detected using the Pierce SuperSignal™ method
|Tested species reactivity||Rat , Human , Mouse , Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide derived from an internal region of the human m-opioid receptor.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The OPRM1 gene encodes the mu opioid receptor, which is the primary site of action for the most commonly used opioids, including morphine, heroin, fentanyl, and methadone. It is also the primary receptor for endogenous opioid peptides beta-endorphin (see POMC, MIM 176830) and the enkephalins (see PENK, MIM 131330). The OPRM1 receptor is a membrane of the G protein-coupled receptor family (Bond et al., 1998 [PubMed 9689128]). There are at least 3 types of opioid receptors, mu, kappa (OPRK1; MIM 165196), and delta (OPRD1; MIM 165195), each with a distinct pharmacologic profile (Chen et al., 1993 [PubMed 8393525]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Familial hemiplegic migraine type 1 mutations W1684R and V1696I alter G protein-mediated regulation of Ca(V)2.1 voltage-gated calcium channels.
44-308G was used in western blot to assess the effects of G protein-dependent modulation on mutations W684R and V696I which cause familial hemiplegic migraine type.
|Garza-López E,Sandoval A,González-Ramírez R,Gandini MA,Van den Maagdenberg A,De Waard M,Felix R||Biochimica et biophysica acta (1822:1238)||2012|
Oprrm1, MORA, LMOR, MOP, muOR, OPRM, Oprm, M-OR-1, MOR-1O, MOR1, MOP-R, mor, MOR, MOR-1
micro opioid receptor isoform hMOR-1A2, mu opiate receptor, mu opioid receptor hMOR-1a, mu-type opioid receptor, M-OR-1, MOR-1, MUOR1, mu opioid receptor splice variant rMOR-1S, mu opioid receptor splice variant rMOR-1Z, opioid receptor B, MOP receptor, mu opioid receptor splice variant mMOR-1Z, OPRM1, LMOR, MOR, Mu-type opioid receptor, HMOP, Opioid mu receptor, MOP, MOR1, Opioid receptor, mu 1, KIAA0403, MOR-1C, Mu opioid receptor hMOR-1a, Opioid mu-type receptor, OPRM