|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic 18 amino acid peptide from C-terminus of human OPRM1 / Mu Opioid Receptor.|
|Purification||Antigen affinity chromatography|
|Contains||< 0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4 - 22 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Hamster, Elephant, Panda, Pig (100%).
The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa , delta, and mu) were first identified by their different selectivities for various naturally occurring alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid sequences of the opioid receptor subtypes are ~70% homologous, and are similar to somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is thought to occur at the third intracellular loop of the opioid receptors, which is also the location of consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the specific receptor subtypes are found on different chromosomes in both the human and mouse genomes. The mu opioid receptor is widely distributed throughout the central nervous system, with higher levels found in the periaqueductal gray region and in the superficial dorsal horn of the spinal cord. The mu receptor demonstrates high affinity to enkephalins and beta-endorphin, and low affinity to dynorphins. The mu opioid receptor is also the high affinity binding protein for the opiate alkaloids such as morphine, codeine, and methadone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.