|Tested species reactivity||Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||ORC2 (hsORC) produced in P3-x63Ag.8.653 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17023 reacts with ORC2 in human samples.
MA5-17023 has been successfully used in immunoprecipitation and Western blot applications.
The MA5-17023 immunogen is ORC2 (hsORC) produced in P3-x63Ag.8.653 cells.
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2L is a subunit of the ORC complex. ORC2L forms a core complex with ORC3L, 4L, and 5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. ORC2L specifically associate with the origin of replication of Epstein Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.