|Tested species reactivity||Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||ORC6 (hsORC) produced in P3-x63Ag.8.653 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17022 reacts with ORC6 in human samples.
MA5-17022 has been successfully used in ChIP, immunoprecipitation and Western blot applications.
The MA5-17022 immunogen is ORC6 (hsORC) produced in P3-x63Ag.8.653 cells.
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC6L is a subunit of the ORC complex. It has been shown that ORC6L and and ORC1L are loosely associated with the core complex consisting of ORC2L, 3L, 4L and 5L. Gene silencing studies with small interfering RNA demonstrated that ORC6L plays an essential role in coordinating chromosome replication and segregation with cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.