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Invitrogen

OTX1/2 Recombinant Rabbit Monoclonal Antibody (5A6)

View all (3) OTX1/2 antibodies

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Product Details

MA573416

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:200
-

Immunoprecipitation (IP)

1:20-1:50
-

ChIP assay (ChIP)

1:20-1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5A6

Immunogen

KLH conjugated synthetic peptide derived from human OTX1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Amount

15 µg

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CRX-related protein; homeobox Otx2; homeobox protein OTX1-like; homeobox protein OTX2-like; homeodomain 159..341; nuclear protein; orthodenticle homolog 1; orthodenticle homolog 2; Otx1; OTX1A; Otx2; unnamed protein product

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Gene Aliases: A730044F23Rik; CPHD6; E130306E05Rik; jv; MCOPS5; OTX1X

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UniProt ID: (Human) P32242, (Human) P32243, (Rat) Q63410, (Mouse) P80205

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Entrez Gene ID: (Human) 5013, (Human) 5015, (Rat) 25646, (Mouse) 18423, (Rat) 305858, (Mouse) 18424

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific double-stranded DNA binding eukaryotic initiation factor 4E binding core promoter proximal region sequence-specific DNA binding
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter anterior/posterior pattern specification metencephalon development forebrain development midbrain development inner ear morphogenesis positive regulation of transcription from RNA polymerase II promoter diencephalon morphogenesis cell fate specification axon guidance central nervous system development endoderm development organ morphogenesis dorsal/ventral pattern formation telencephalon regionalization cell differentiation neuron differentiation somite rostral/caudal axis specification positive regulation of embryonic development eye photoreceptor cell fate commitment cell fate commitment positive regulation of transcription, DNA-templated neuron fate commitment neuron fate determination oligodendrocyte differentiation protein-containing complex assembly dopaminergic neuron differentiation primitive streak formation cellular response to leukemia inhibitory factor positive regulation of gastrulation regulation of smoothened signaling pathway regulation of fibroblast growth factor receptor signaling pathway
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