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Immunofluorescent analysis of Occludin in U2OS cells using an Occludin recombinant rabbit monoclonal antibody (Product # 701161) followed by detection using an Alexa Fluor 594 goat anti-rabbit secondary antibody (red) (Image A). Image B is a composite image with nuclei stained using DAPI (blue) showing localization of occludin at tight junctions.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 387–403 of human occludin|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tight junction group of proteins are a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junction strands are mainly composed of claudins, occludin, and JAM. Occludin is a 65 kDa protein and is considered to be vital in the assembly and maintenance of tight junctions. At the tight junction, occludin associates with membrane peripheral protein ZO-1. The expression of occludin varies greatly on the type of tissue. It is continuously and highly expressed in brain whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Claudin 1 expression characterizes human uterine cervical reserve cells.
||Zinner B,Gyöngyösi B,Babarczi E,Kiss A,Sobel G||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (61:880)||2013|
Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
||Eker B,Meissner R,Bertsch A,Mehta K,Renaud P||PloS one (8:null)||2013|
Distinguishing drug-induced minor morphological changes from major cellular damage via label-free impedimetric toxicity screening.
||Meissner R,Eker B,Kasi H,Bertsch A,Renaud P||Lab on a chip (11:2352)||2011|
occludin, tight junction protein occludin, OCLN