|Western Blot (WB)||1:500-1:5000|
|Western Blot (WB)||See 2 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Pig Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 387–403 of human occludin|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tight junction group of proteins are a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junction strands are mainly composed of claudins, occludin, and JAM. Occludin is a 65 kDa protein and is considered to be vital in the assembly and maintenance of tight junctions. At the tight junction, occludin associates with membrane peripheral protein ZO-1. The expression of occludin varies greatly on the type of tissue. It is continuously and highly expressed in brain whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Occludin; OCLN; phosphatase 1, regulatory subunit 115; tight junction protein occludin
Gene Aliases: BLCPMG; OCLN; PPP1R115
UniProt ID: (Human) Q16625
Entrez Gene ID: (Human) 100506658
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