Invitrogen

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 594

Product Image Gallery

Occludin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Occludin Antibody, Alexa Fluor® 594 conjugate (OC-3F10) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody, Alexa Fluor® 594 conjugate (OC-3F10) (Product # 331594) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c: red). Panel c is a merged image showing cell junctional localization. Panel d is a no primary antibody control. The images were captured at 40X magnification.

Occludin Antibody in Western Blot (WB) — Figure 3 Western blot analysis of epithelial cell protein and cell proliferation receptor protein expression. Changes in the expression level of the epithelial cell protein ( A , B ), proliferation receptor protein ( C , D ), ECM/ Cell tight junction protein ( E , F ) as determined by western blot analysis. Increased expression of E-cadherin, p-AKT, p-Erk, Fibronectin, ZO-1, and Occludin under ASM culture conditions. All factors were normalized to beta-actin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Product Details

331594

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2.5-5 µg/mL	View 5 publications 5 publications
ELISA (ELISA)	0.1-1 µg/mL	-

Product Specifications
Species Reactivity	Dog, Human, Mouse, Rat
Published species	Dog, Human, Mouse, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	OC-3F10
Immunogen	GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin. View immunogen
Conjugate	Alexa Fluor™ 594 Alexa Fluor 488 FITC HRP Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 4mg/mL BSA
Contains	0.1% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_2532186

Product Specific Information

Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and Western blotting (un-conjugated). Tissues/lysates tested: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse) and rat liver This antibody reacts specifically with mammalian occludin.

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Three-Dimensional Aggregated Spheroid Model of Hepatocellular Carcinoma Using a 96-Pillar/Well Plate.

Molecules (Basel, Switzerland)

Lee SY,Teng Y,Son M,Ku B,Hwang HJ,Tergaonkar V,Chow PK,Lee DW,Nam DH

331594 was used in Western Blot to we proposed a 3D aggregated spheroid model (ASM) to create large single spheroids by aggregating cells in Matrigel attached to the surface of 96-pillar plates during cell culture.

Mon Aug 16 00:00:00 EDT 2021

Three-Dimensional Aggregated Spheroid Model of Hepatocellular Carcinoma Using a 96-Pillar/Well Plate.

Molecules (Basel, Switzerland)

Lee SY,Teng Y,Son M,Ku B,Hwang HJ,Tergaonkar V,Chow PK,Lee DW,Nam DH

Mon Aug 16 00:00:00 EDT 2021

Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model.

Journal of fungi (Basel, Switzerland)

Luvanda MK,Posch W,Noureen A,Lafon E,Zaderer V,Lass-Flörl C,Wilflingseder D

331594 was used in Immunocytochemistry to show that corticosteroids promote enhanced fungal growth and invasion A. fumigatus by creating a suppressive environment affecting both epithelial as well as immune cells.

Thu Mar 18 00:00:00 EDT 2021

The Na+, K+-ATPase β1 subunit regulates epithelial tight junctions via MRCKα.

JCI insight

Bai H,Zhou R,Barravecchia M,Norman R,Friedman A,Yu D,Lin X,Young JL,Dean DA

331594 was used in Immunocytochemistry to elucidate a novel mechanism by which NKA regulates epithelial tight junctions and identified potential drug targets for treating ARDS and other pulmonary diseases that are caused by barrier dysfunction.

Mon Feb 22 00:00:00 EST 2021

The Na+, K+-ATPase β1 subunit regulates epithelial tight junctions via MRCKα.

JCI insight

Bai H,Zhou R,Barravecchia M,Norman R,Friedman A,Yu D,Lin X,Young JL,Dean DA

Mon Feb 22 00:00:00 EST 2021

Occludin, caveolin-1, and Alix form a multi-protein complex and regulate HIV-1 infection of brain pericytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Torices S,Roberts SA,Park M,Malhotra A,Toborek M

331594 was used in Immunocytochemistry to identify a novel mechanism involved in HIV-1 infection contributing to a better understanding of the HIV-1 pathology and the associated neuroinflammatory responses.

Tue Dec 01 00:00:00 EST 2020

Occludin, caveolin-1, and Alix form a multi-protein complex and regulate HIV-1 infection of brain pericytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Torices S,Roberts SA,Park M,Malhotra A,Toborek M

Tue Dec 01 00:00:00 EST 2020

Asymmetric Stratification-Induced Polarity Loss and Coordinated Individual Cell Movements Drive Directional Migration of Vertebrate Epithelium.

Cell reports

Lu Y,Deng R,You H,Xu Y,Antos C,Sun J,Klein OD,Lu P

331594 was used in Immunocytochemistry to show that directional migration of the mammary epithelium is a unique multistep process and that, despite sharing remarkable cellular and molecular similarities, vertebrate and invertebrate epithelial branching are fundamentally distinct processes.

Tue Oct 13 00:00:00 EDT 2020

Maternal administration of probiotics promotes gut development in mouse offsprings.

PloS one

Yu Y,Lu J,Oliphant K,Gupta N,Claud K,Lu L

331594 was used in Immunohistochemistry to investigate if maternal administration of probiotics to pregnant mouse dams can recapitulate the beneficial effects observed in neonates fed with probiotics directly.

Thu Oct 08 00:00:00 EDT 2020

Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier.

Nature communications

Campos Y,Qiu X,Gomero E,Wakefield R,Horner L,Brutkowski W,Han YG,Solecki D,Frase S,Bongiovanni A,d'Azzo A

331594 was used in Immunocytochemistry-immunoflourescence to examine the role of Alix in the regulation of the spatial organisation of the actin cytoskeleton and proper positioning/assembly of intercellular junctions.