Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Occludin Antibodies

          Invitrogen

          Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 594

          3 Published Figures
          10 References
          View all (59) Occludin antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 594

          • Antibody Testing Data (2)
          • Published Figures (3)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Occludin Antibody (331594) in ICC/IF

          Immunofluorescence analysis of Occludin Antibody, Alexa Fluor® 594 conjugate (OC-3F10) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody, Alexa Fluor® 594 conjugate (OC-3F10) (Product # 331594) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Western Blot (WB)

          Product Details

          331594

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          2.5-5 µg/mL
          View 7 publications 7 publications

          ELISA (ELISA)

          0.1-1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Published species

          Dog, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OC-3F10

          Immunogen

          GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.
          View immunogen

          Conjugate

          Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594
          • Unconjugated
          • Alexa Fluor 488 
          • FITC
          • HRP
          • Request custom conjugation

          Excitation/Emission Max

          590/618 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 4mg/mL BSA

          Contains

          0.1% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2532186

          Product Specific Information

          Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and Western blotting (un-conjugated). Tissues/lysates tested: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse) and rat liver This antibody reacts specifically with mammalian occludin.

          Target Information

          Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein

          View more View less

          Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115

          View more View less

          UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5

          View more View less

          Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497

          View more View less

          Function(s)
          protein binding thiopurine S-methyltransferase activity protein domain specific binding S-adenosylmethionine-dependent methyltransferase activity structural molecule activity general transcription factor
          Process(es)
          positive regulation of lamellipodium assembly positive regulation of microtubule polymerization bicellular tight junction assembly positive regulation of wound healing protein localization to cell leading edge protein complex assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-green-56756dfb8b-mfpfg:80/100.66.79.199:80.
          git-commit: de6905f249d25e22729e613aef254bb544bd8b7c
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.1-2025.06.25-1.0