Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-1 µg/mL | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
2.5-5 µg/mL | View 5 publications 5 publications |
ELISA (ELISA) |
0.1-1 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Human, Mouse, Rat |
Published species |
Dog, Human, Mouse, Rat |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OC-3F10 |
Immunogen |
GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin. |
Conjugate |
Alexa Fluor™ 594
|
Excitation/Emission Profile |
View spectra |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4, with 4mg/mL BSA |
Contains |
0.1% sodium azide |
Storage conditions |
4° C, store in dark |
RRID |
AB_2532186 |
Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and Western blotting (un-conjugated). Tissues/lysates tested: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse) and rat liver This antibody reacts specifically with mammalian occludin.
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein
Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115
UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5
Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497
Molecular Function:
general transcription factor
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