Immunofluorescent analysis of Oct4/Pou5F1 (green) shows nuclear staining in human HEL 11.4 iPS cells, indicating pluripotency. Oct4/Pou5F1 staining (left panel), DAPI staining (middle panel) and an overlay image of Oct4/Pou5F1 with DAPI (right panel) are shown. HEL 11.4 iPS cells were grown on matrigel-coated chamber slides and fixed with 4% formaldehyde for 15 minutes prior to staining. Cells were probed with a DyLight 488-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-D488) in 0.1% Tween-PBS at a dilution of 1:20, and incubated overnight at 4C. Images were taken at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full length human recombinant protein of human POU5F1 produced in E.coli.|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-104-D488 detects Oct4/Pou5F1 in human iPS samples.
MA1-104-D488 has been successfully used in ICC/IF applications.
Oct4 is a transcription factor which plays a key role in embryonic development, especially during early embryogenesis. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.