Alomone Labs, LTD

Orai1 (extracellular) Monoclonal Antibody (3F11/D10/B9), Biotin

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Orai1 (extracellular) Antibody in Western Blot (WB) — Western blot analysis using Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (1:500): - 1. Jurkat cells. 2. HEK cells transfected with human Orai1.3. HEK cells transfected with control vector.

Orai1 (extracellular) Antibody in Flow Cytometry (Flow) — Cell surface detection of Orai1 in intact living Jurkat cells: - (black) Unstained cells. (red) Cells + Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (5-10 µg Antibody /0.5-1 x 106cells).

Product Details

ALM-025-B-50UG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Flow Cytometry (Flow)	5 µg/0.5-1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgM
Class	Monoclonal
Type	Antibody
Clone	3F11/D10/B9
Immunogen	Synthetic mapping to an extracellular sequence of human Orai1, extracellular sequence of human Orai1 (106-119, 195-234). View immunogen
Conjugate	Biotin Atto 633 Unconjugated
Form	Lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 50 mcg double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

ORAI1 is a plasma membrane protein essential for store-operated calcium entry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: calcium release-activated calcium channel 1; Calcium release-activated calcium channel protein 1; calcium release-activated calcium modulator 1; FLJ14466; Protein orai-1; Transmembrane protein 142A

Gene Aliases: CRACM1; D730049H07Rik; IMD9; orai-1; ORAI1; ORAT1; TAM2; TMEM142A

UniProt ID: (Human) Q96D31, (Mouse) Q8BWG9

Entrez Gene ID: (Human) 84876, (Mouse) 109305

Molecular Function: ion channel transporter