Immunofluorescent analysis of Orphanin FQ using an Orphanin FQ polyclonal antibody (Product # PA1-30933). Confocal images showing OFQ (red), GnRH (green) and their colocalization (yellow) in the ovine preoptic area and hypothalamus. A-C: Low power view of the preoptic area at the level of the organum vasculosum of the lamina terminalis (OVLT) showing OFQ and GnRH cells, and their colocalization (e.g., white arrows). Bar = 100µm. D-F: High power images of a double-labeled OFQ/GnRH cell; note that the immunofluorescence for OFQ has a punctateappearance in the cytoplasm as opposed to the more diffuse appearance of the GnRH signal. Bar 26 = 20µm. G-I: Single-labeled OFQ cells (e.g., red arrow) in the arcuate nucleus. Note that many GnRH fibers coursing through this region are also OFQ-positive (e.g., white arrows). 3v = third ventricle. Bar = 100µm.
|Tested species reactivity||Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FGGFTGARKSARKLANQ Corresponding to residues 122-138 of rat Orphanin FQ|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30933 detects Orphanin FQ from rat, non-human primate samples.
PA1-30933 has been successfully used in immunohistochemistry applications.
The PA1-30933 immunogen is: FGGFTGARKSARKLANQ Corresponding to residues 122-138 of rat Orphanin FQ
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.