|Tested species reactivity||Bovine, Human|
|Published species reactivity||Dog, Rat, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine Osteocalcin|
|Purification||Ammonium sulfate precipitation, Ion-exchange chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Osteocalcin (Bone Gla Protein) is a 49 amino acid single chain vitamin K dependent protein (MW 5.8 kDa), made by osteoblast that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increase bone turnover such as osteoporosis, hyperparathyroidism, and Paget's disease, and low condition asssociated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts.
33-5400 was used in immunohistochemistry to elucidate the behavior of canine bone marrow stromal cells during differentiation into osteoblasts utilizing different implant biomaterials
|Özdal-Kurt F,Tu¿lu I,Vatansever HS,Tong S,¿en BH,Delilo¿lu-Gürhan SI||Biotechnic and histochemistry : official publication of the Biological Stain Commission (91:412)||2016|
The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction.
33-5400 was used in immunohistochemistry to assess the use of cultured autologous bone marrow stromal cells on different scaffolds for healing defects in the tibias of dogs.
|Özdal-Kurt F,Tu¿lu I,Vatansever HS,Tong S,Delilo¿lu-Gürhan SI||Biotechnic and histochemistry : official publication of the Biological Stain Commission (90:516)||2015|
|Not Applicable||Not Cited||
Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium.
33-5400 was used in immunocytochemistry to characterize the growth and attachment of dental pulp stem cells on dentin with extra calcium
|Özdal-Kurt F,¿en BH,Tu¿lu I,Vatansever S,Türk BT,Delilo¿lu-Gürhan I||Archives of oral biology (68:131)||2016|
|Rat||Not Cited||Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study.||Oztürk F,Babacan H,Inan S,Gümü¿ C||American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics (140:e31)||2011|