|Tested species reactivity||Bovine, Dog, Goat, Human, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Full length, bovine osteocalcin protein.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-10 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
MA1-20786 detects osteocalcin in human, rat, bovine, canine, porcine, sheep, goat, and rabbit samples. This antibody does not cross react with mouse Osteocalcin.
MA1-20786 has been successfully used in ELISA, immunohistochemistry (frozen and paraffin), and Western blot procedures. Predicted molecular weight of 12 kDa.
The MA1-20786 immunogen is full length, bovine osteocalcin protein. This antibody binds an epitope containing gamma-carboxylated residue 17.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Experimental Transmission of Bighorn Sheep Sinus Tumors to Bighorn Sheep (Ovis canadensis canadensis) and Domestic Sheep.
MA1-20786 was used in immunohistochemistry - paraffin section to characterize the experimental transmission of bighorn sheep sinus tumors to domestic sheep and bighorn sheep (ovis canadensis canadensis)
|Fox KA,Wootton S,Marolf A,Rouse N,LeVan I,Spraker T,Miller M,Quackenbush S||Veterinary pathology (53:1164)||2016|