|Tested species reactivity||Bovine, Dog, Goat, Human, Sheep, Pig, Rabbit, Rat|
|Host / Isotype||Mouse / IgG3|
|Immunogen||Full length bovine protein.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25584 detects Osteocalcin from human, rat, bovine, canine, goat, porcine, rabbit, and ovine samples. MA1-25584 will not react with mouse.
MA1-25584 has been successfully used in immunohistochemistry (frozen / paraffin) and Western blot procedures.
The MA1-25584 immunogen is full length bovine protein. This antibody detects an epitope corresponding to residues 21-31 of bovine Osteocalcin.
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.