Immunohistochemistry of bone substitute stained for Osteocalcin using Anti-Osteocalcin Monoclonal Antibody (MA1-20788). Bone substitute (hydroxyapatite and silicon) was implanted subcutaneously into sheep for 12 weeks. After a series of deparaffinization, rehydration, and blocking steps, paraffin-embedded bone sections were stained with MA1-20788 (1:10 diluted in 10% normal goat serum in 1X PBS) for 3 hours, followed by a biotin-conjugated anti-mouse IgG secondary antibody for 1.5 hours, an avidin-biotin complex reagent for 1 hour, and finally DAB substrate. Sections were counterstained with hematoxylin. Osteocalcin staining is shown in brown, and nuclear staining is shown in blue. Magnification = 100X. Data courtesy of the Innovators Program.
|Tested species reactivity||Bovine, Dog, Goat, Human, Sheep, Pig, Rabbit|
|Published species reactivity||Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length, bovine osteocalcin protein.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/mL|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-20788 detects Osteocalcin in human, bovine, ovine, canine, porcine, goat, and rabbit samples. This antibody does not detect rat or mouse Osteocalcin.
MA1-20788 has been successfully used in ELISA, immunohistochemistry (frozen and paraffin), and Western blot procedures. Predicted molecular weight: 12kDa.
The MA1-20788 immunogen is full length, bovine osteocalcin protein.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of pathological differentiation of valvular interstitial cells by C-type natriuretic peptide.
MA1-20788 was used in immunocytochemistry to investigate the inhibitory effect of CNP on valvular interstitial cells
|Yip CY,Blaser MC,Mirzaei Z,Zhong X,Simmons CA||Arteriosclerosis, thrombosis, and vascular biology (31:1881)||2011|