Description: The 2F10 monoclonal antibody recognizes human osteopontin (OPN), a secreted glycophosphoprotein expressed by multiple cell types, including leukocytes, fibroblasts, dendritic cells, macrophages, myoblasts, bone-forming cells, and epithelial cells. OPN is an extracellular matrix protein with multiple roles, including bone resorption and calcification, immune system regulation, wound healing, and tumorigenesis. Thrombin cleavage of OPN allows binding of integrin receptors, expressed by immune cells, and subsequent modulation of cell adhesion, migration, and survival. OPN is expressed in bones, teeth, placenta, blood vessels, kidney, and various tumors. Osteopontin has been identified as a potential therapeutic target in the treatment of autoimmune disease, cancer metastasis, bone mineralization diseases, and osteoporosis.
Applications Reported: This 2F10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2F10 antibody has been pre-titrated and tested. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
Protein Aliases: Bone sialoprotein 1; early T-lymphocyte activation 1; HGNC:11255; MGC110940; Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin
Gene Aliases: BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1
UniProt ID: (Human) P10451
Entrez Gene ID: (Human) 6696