|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin.|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with dog, mouse and rat based on sequence homology.
Western blot detects a band of approximately 60 kDa in lysates of human thymus, tonsil and spleen.
Osteopontin is an acid-rich, multidomain, phosphorylated 60kDa secreted glycoprotein that is a member of the SIBLING family, and is synthesised by many cell types. Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium. Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.