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|Tested species reactivity||Dog, Human, Pig|
|Published species reactivity||Dog, Sheep, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from internal domain of human Osteopontin|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16821 targets Osteopontin in IHC (P) and WB applications and shows reactivity with Canine, Human, and Porcine samples.
The PA5-16821 immunogen is a synthetic peptide derived from internal domain of human Osteopontin.
Osteopontin is a 34kDa extracellular matrix protein with a cell-binding domain. It was originally identified as a major component of the non-collagenous organic bone matrix however it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis. This antibody is suitable for staining of formalin-fixed, paraffin-embedded tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Smad7 regulates terminal maturation of chondrocytes in the growth plate.
PA5-16821 was used in immunohistochemistry to study the role of Smad7 in modulating chondrocyte terminal maturation at the growth plate using knock out mice
|Estrada KD,Wang W,Retting KN,Chien CT,Elkhoury FF,Heuchel R,Lyons KM||Developmental biology (382:375)||2013|
Initial pulmonary respiration causes massive diaphragm damage and hyper-CKemia in Duchenne muscular dystrophy dog.
PA5-16821 was used in immunohistochemistry to study the massive diaphragm damage and elevated serum creatine kinase levels resulting from the initial pulmonary respiration in a canine model of Duchenne muscular dystrophy
|Nakamura A,Kobayashi M,Kuraoka M,Yuasa K,Yugeta N,Okada T,Takeda S||Scientific reports (3:null)||2013|
A new piezoelectric actuator induces bone formation in vivo: a preliminary study.
PA5-16821 was used in immunohistochemistry to study the use of a novel piezoelectric device to mechanically stimulate bone formation
|Reis J,Frias C,Canto e Castro C,Botelho ML,Marques AT,Simões JA,Capela e Silva F,Potes J||Journal of biomedicine and biotechnology (2012:null)||2012|
beta-Tricalcium phosphate in the early phase of socket healing: an experimental study in the dog.
PA5-16821 was used in immunohistochemistry to investigate the role of beta-Tricalcium phosphate in the process of socket healing
|Araújo MG,Liljenberg B,Lindhe J||Clinical oral implants research (21:445)||2010|
Dynamics of Bio-Oss Collagen incorporation in fresh extraction wounds: an experimental study in the dog.
PA5-16821 was used in immunohistochemistry to study the therapeutic benefit of Bio-Oss Collagen in dental wound healing
|Araújo MG,Liljenberg B,Lindhe J||Clinical oral implants research (21:55)||2010|
Downregulation of protein tyrosine phosphatase PTPL1 alters cell cycle and upregulates invasion-related genes in prostate cancer cells.
PA5-16821 was used in western blot to study the role of PTPL1 in regulating prostate cancer cell proliferation and invasiveness
|Castilla C,Flores ML,Conde JM,Medina R,Torrubia FJ,Japón MA,Sáez C||Clinical and experimental metastasis (29:349)||2012|
Bone sialoprotein 1; bone sialoprotein I; bone sialoprotein); early T-lymphocyte activation 1; early T-lymphocyte activation 1); Nephropontin; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; secreted phosphoprotein-1 (osteopontin; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin
BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1