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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length mouse protein, expressed in mouse NSO cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24881 detects Osteoprotegerin from mouse samples. PA1-24881 is expected to cross react with human (89% conserved) and rat (93% conserved) due to sequence homology.
PA1-24881 has been successfully used in neutralization, inhibition assays, and Western blot procedures. For Western blotting, use antibody in non-reducing conditions. The Neutralization Dose50 (ND50) for anti-mouse osteoprotegerin is 0.5-2 ug/ml in the presence of 0.1 ug/ml of soluble recombinant mouse OPG/Fc, using the TRAIL sensitive mouse L929 cytolytic assay.
The PA1-24881 immunogen is recombinant, full length mouse protein, expressed in mouse NSO cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
osteoclastogenesis inhibitory factor; Osteoprotegerin; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b
OCIF; OPG; PDB5; TNFRSF11B; TR1