|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length mouse protein, expressed in mouse NSO cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24881 detects Osteoprotegerin from mouse samples. PA1-24881 is expected to cross react with human (89% conserved) and rat (93% conserved) due to sequence homology.
PA1-24881 has been successfully used in neutralization, inhibition assays, and Western blot procedures. For Western blotting, use antibody in non-reducing conditions. The Neutralization Dose50 (ND50) for anti-mouse osteoprotegerin is 0.5-2 ug/ml in the presence of 0.1 ug/ml of soluble recombinant mouse OPG/Fc, using the TRAIL sensitive mouse L929 cytolytic assay.
The PA1-24881 immunogen is recombinant, full length mouse protein, expressed in mouse NSO cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.