Western blot analysis of human neuroblastoma cell line whole cell lysate using PA5-19841, Osteoprotegerin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19841 staining of K562 whole cell lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 200 - 300 Osteoprotegerin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 105 kDa and 14 kDa.
This antibody is predicted to react with mouse, sheep, cow and pig based on sequence homology.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.