|Western Blot (WB)||6 ug/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / Ig Mix|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
This product is a cocktail of multiple OxPhos antibodies.
C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
C-IV-I (clone) 1D6E1A8 - Human Complex IV subunit I
C-V-alpha (clone) 15H4C4 - Bovine Complex V
The kit is suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from mouse, rat, human, or bovine sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Oxidative Phosphorylation; OxPhos Complex
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.