|Tested species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.|
|Storage buffer||PBS with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5-10 µg for 1x10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-10 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26528 detects P Glycoprotein from human, hamster, mouse and rat samples. MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.
MA1-26528 has been successfully used in immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, and Western blot procedures.
The MA1-26528 immunogen is SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line. This antibody recognizes a highly conserved internal sequence (VQEALD and VQAALD) corresponding to the C- and N-terminal regions, respectively, from both MDR1 and MDR3 isoforms of P Glycoprotein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
P-gp efflux pump inhibition potential of common environmental contaminants determined in vitro.
MA1-26528 was used in immunocytochemistry to study the ability of common aquatic environmental contaminants to inhibit MDR-1
|Georgantzopoulou A,Skoczy¿ska E,Van den Berg JH,Brand W,Legay S,Klein SG,Rietjens IM,Murk AJ||Environmental toxicology and chemistry (33:804)||2014|
Protein phosphatase complex PP5/PPP2R3C dephosphorylates P-glycoprotein/ABCB1 and down-regulates the expression and function.
MA1-26528 was used in western blot to investigate the relationship between PP5/PPP2R3C and P-gp
|Katayama K,Yamaguchi M,Noguchi K,Sugimoto Y||Cancer letters (345:124)||2014|
Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome-microbubble complexes assisted by ultrasound.
MA1-26528 was used in western blot to study the ability of novel doxorubicin liposome complexes plus ultrasound to overcome the multidrug resistance of human breast cancer cells
|Deng Z,Yan F,Jin Q,Li F,Wu J,Liu X,Zheng H||Journal of controlled release : official journal of the Controlled Release Society (174:109)||2014|
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
MA1-26528 was used in western blot to study cord blood as a source of circulating endothelial progenitor cells that can be directed towards specialized endothelial phenotypes
|Boyer-Di Ponio J,El-Ayoubi F,Glacial F,Ganeshamoorthy K,Driancourt C,Godet M,Perrière N,Guillevic O,Couraud PO,Uzan G||PloS one (9:null)||2014|