Immunofluorescent analysis of p170 (green) showing staining in the membrane of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p170 monoclonal antibody (Product # MA5-13854) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Hamster, Human|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human and hamster drug-resistant viable cells followed by crude plasma membranes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13854 targets p170 in FACS, WB and ICC/IF applications and shows reactivity with Hamster and Human samples. This antibody does not react with mouse tissue in Western blot applications.
The MA5-13854 immunogen is human and hamster drug-resistant viable cells followed by crude plasma membranes.
Multi-drug resistance(MDR) associated p-glycoprotein (p170) causes resistance to various drugs in a number of cancer patients. It exists in a soluble and membrane associated form. Soluble P-glycoprotein is detected in extracellular fluids of cancer patients, such as malignant ascites and serum. The Mr of soluble P-glycoprotein is the same as that of membrane bound P-glycoprotein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
MA5-13854 was used in immunocytochemistry to describe methods to differentiate and characterize hPSC-derived brain microvascular endothelial cells
|Stebbins MJ,Wilson HK,Canfield SG,Qian T,Palecek SP,Shusta EV||Methods (San Diego, Calif.) (101:93)||2016|
Influence of P-glycoprotein on brucine transport at the in vitro blood-brain barrier.
MA5-13854 was used in immunocytochemistry to study the ability of P-glycoprotein to prevent the transport of brucine through an in vitro blood-brain barrier
|Xu DH,Yan M,Li HD,Fang PF,Liu YW||European journal of pharmacology (690:68)||2012|
ABCB1 is predominantly expressed in human fetal neural stem/progenitor cells at an early development stage.
MA5-13854 was used in immunocytochemistry and immunohistochemistry to study the expression of the ABCB1 transporter in human fetal neural stem/progenitor cells
|Yamamoto A,Shofuda T,Islam MO,Nakamura Y,Yamasaki M,Okano H,Kanemura Y||Journal of neuroscience research (87:2615)||2009|
Effect of the novel antipsychotic drug perospirone on P-glycoprotein function and expression in Caco-2 cells.
MA5-13854 was used in immunocytochemistry to study the effects of the novel anti-psychotic drug perospirane of the expression and pump activity of P-glycoprotein
|Zhou YG,Li KY,Li HD||European journal of clinical pharmacology (64:697)||2008|
[The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis].
MA5-13854 was used in immunohistochemistry to examine the prognostic significance of multiple genes in osteosarcoma
|Ozger H,Eralp L,Atalar AC,Toker B,Esberk Ate¿ L,Sungur M,Bilgiç B,Ayan I||Acta orthopaedica et traumatologica turcica (43:28)||2009|
Efficacy of Hsp90 inhibition for induction of apoptosis and inhibition of growth in cervical carcinoma cells in vitro and in vivo.
MA5-13854 was used in western blot to study the effect of Hsp90 inhibitors on cervical carcinoma survival and growth in vitro and in vivo
|Schwock J,Pham NA,Cao MP,Hedley DW||Cancer chemotherapy and pharmacology (61:669)||2008|
Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.
MA5-13854 was used in western blot to investigate the role of HBx protein on the occurrence of multidrug resistance in hepatoma cell line
|Guan J,Chen XP,Zhu H,Luo SF,Cao B,Ding L||World journal of gastroenterology (10:3522)||2004|
Reversal of P-glycoprotein-mediated multidrug resistance with small interference RNA (siRNA) in leukemia cells.
MA5-13854 was used in western blot to study the ability of siRNA targeted against P-glycoprotein to reverse multi-drug resistance in a human leukemia cell line
|Peng Z,Xiao Z,Wang Y,Liu P,Cai Y,Lu S,Feng W,Han ZC||Cancer gene therapy (11:707)||2004|
P-glycoprotein expression and distribution in the rat placenta during pregnancy.
MA5-13854 was used in western blot to study the expression and distribution of P-glycoprotein in the rat placenta during pregnancy
|Novotna M,Libra A,Kopecky M,Pavek P,Fendrich Z,Semecky V,Staud F||Reproductive toxicology (Elmsford, N.Y.) (18:785)||2004|
ABCG2 overexpression in colon cancer cells resistant to SN38 and in irinotecan-treated metastases.
MA5-13854 was used in western blot to study the role of ABCG2 overexpression in the development of irinotecan resistance in colon cancer cells
|Candeil L,Gourdier I,Peyron D,Vezzio N,Copois V,Bibeau F,Orsetti B,Scheffer GL,Ychou M,Khan QA,Pommier Y,Pau B,Martineau P,Del Rio M||International journal of cancer (109:848)||2004|
Examination of the functional activity of P-glycoprotein in the rat placental barrier using rhodamine 123.
MA5-13854 was used in western blot to study the functional activity of P-glycoprotein in the rat placental barrier
|Pavek P,Staud F,Fendrich Z,Sklenarova H,Libra A,Novotna M,Kopecky M,Nobilis M,Semecky V||The Journal of pharmacology and experimental therapeutics (305:1239)||2003|
Differences in multidrug resistance phenotype and matrix metalloproteinases activity between endothelial cells from normal brain and glioma.
MA5-13854 was used in western blot to study differences in the expression of multidrug resistance pumps and MMP activity in endothelial cells from normal brain and glioma
|Régina A,Demeule M,Bérubé A,Moumdjian R,Berthelet F,Béliveau R||Journal of neurochemistry (84:316)||2003|
Dexamethasone modulation of multidrug transporters in normal tissues.
MA5-13854 was used in western blot to study the effect of dexamethasone on multidrug drug transporter expression in normal rat tissues
|Demeule M,Jodoin J,Beaulieu E,Brossard M,Béliveau R||FEBS letters (442:208)||1999|