Invitrogen

P-cadherin Monoclonal Antibody (PCD-1)

Product Image Gallery

P-cadherin Antibody — Figure 6. P-cadherin expression is sufficient to induce MC dedifferentiation and hyperproliferation. (A) TRP2 immunostaining of back skin cross sections of P0 control and E-cad eKO mice. Data are representative of three independent experiments. (B) Quantification of MC number in interfollicular epidermis of control and E-cad eKO P0 back skin. Data are pooled and represented as mean +- SD. n = 3 mice per group. *, P = 0.0133; Student and apos;s t test. (C) Western blot analysis for P- and E-cadherin expression in control, E-cad KO (KO), and E-cad KO /P-cad KD (KK) KCs. Data are representative of three independent experiments. (D) Phase-contrast micrographs of MCs co-cultured with control (left), E-cad KO (middle), or E-cad KO / P-cad KD (right) KCs for 3 d at HC. Data are representative of three independent experiments. (E) Quantification of D (eight images per condition). Data are pooled and represented as mean +- SD. Dendritic length: n = 3 biological replicates per group; Ctrl versus KO: ***, P = 0.0003; KO versus KK: ***, P = 0.0005; one-way ANOVA with Tukey posthoc test. MC numbers: n = 3 biological replicates per group; Ctrl versus KO: **, P = 0.0038; KO versus KK: **, P = 0.0042; one-way ANOVA with Tukey posthoc test. (F) Western blot analysis for P-cadherin in control- and P-cadherin-expressing CHOs. Data are representative of two independent experiments. (G) Quantification of MC number co-cultured with control or P-cadherin overexpressing CHO cells for 4 d at HC (20 image

Product Details

Tested Applications

Dilution

Inhibition Assays (IA)

60 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

Assay Dependent

Western Blot (WB)

10 µg/mL

Published Applications

Immunofluorescence (IF)

See 8 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 3 publications below

Western Blot (WB)

See 2 publications below

Immunohistochemistry (IHC)

See 5 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 2 publications below

Flow Cytometry (Flow)

See 1 publication below

In vitro Assay (IV)

See 1 publication below

Miscellaneous PubMed (MISC)

See 4 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Bovine, Fish, Hamster, Human, Mouse, Rat, Sheep, Tag

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PCD-1

Immunogen

Mouse endoderm cell line PSA5-E.

Conjugate

Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

10mM PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4° C

RRID

AB_2533006

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell-line with spleen cells of Donryu rat after immunization with mouse endoderm cell line PSA5-E.

Specificity: This antibody specifically reacts with mouse P-cadherin. This antibody inhibits P-cadherin-dependent cell-cell contact.

Cross reactivity: This antibody does not react with human or chicken P-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µl or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

Target Information

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cadherin-3; calcium-dependent adhesion protein; CDHP; HJMD; P-cadherin; PCAD; placental; Placental cadherin; RPE-specific cadherin

Gene Aliases: AI385538; Cadp; Cdh3; Cdhp; P-cadherin; Pcad

UniProt ID: (Mouse) P10287

Entrez Gene ID: (Mouse) 12560

Molecular Function: cadherin