Invitrogen

P-cadherin Monoclonal Antibody (PCD-1)

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P-cadherin Antibody in Immunocytochemistry (ICC/IF) — Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunocytochemistry

P-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Figure 7. Reduced epidermal PAR3 and increased P-cadherin levels at heterologous contacts correlate with progression of human melanoma. (A) H&E staining (top) and PAR3 and TRP2 immunostainings (bottom) of human normal skin and melanoma tissue. Bars: (top) 100 um; (bottom) 20 um. Data are representative of at least three independent experiments. (B) Quantification of epidermal anti-PAR3 immunoreactivity in normal skin epidermis ( n = 10 tissues from different donors), melanoma with tumor depth ~1 mm ( n = 9 tumors from different patients), and melanoma with tumor depth ~10 mm ( n = 8 tumors from different patients). Data are mean + SEM. *, P = 0.0113; **, P = 0.0040; one-way ANOVA with Tukey posthoc test. (C) P-cadherin and TRP2 costaining in melanoma in situ and melanoma with ~10 mm depth revealing high levels of P-cadherin at sites of KC-MC contacts. Bar, 10 um. Data are representative of at least three independent experiments. (D) P-cadherin immunostainings of human normal skin and melanoma tissue. Bar, 20 um. Data are representative of at least three independent experiments. (E) Quantification of anti-P-cadherin immunoreactivity in melanoma patients in KC-MC contacts of normal skin epidermis ( n = 9 tissues from different donors), melanoma in situ ( n = 9 tissues from different patients), melanoma with tumor depth ~1 mm ( n = 8 tumors from different patients), and ~10-mm melanoma ( n = 4 tumors from different patients). Data are pooled and depicted as mean + SEM. **, P = 0

P-cadherin Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P)) — Figure 4. Par3 loss in KCs increases P-cadherin surface levels and stability and enforces P-cadherin localization to heterologous KC-MC contacts. (A) P-cadherin immunostaining in Par3 KO KC-MC co-culture and MC monoculture. Bar, 10 um. (B) P-cadherin immunostaining in control and Par3 KO co-cultures. Bar, 10 um. (C) P-cadherin and Par3 coimmunostainings of neonatal control skin cross sections depicting interfollicular epidermis (top) and hair follicle (bottom) areas. Dashed lines demarcate the MC-containing basal epidermal layer. Bar, 10 um. (D) P-cadherin and TRP2 immunohistochemistry of neonatal skin cross sections. Dashed lines underline the MC-containing basal epidermal layer. Bar, 10 um. (A-D) Data are representative of three independent experiments. (E) Quantification of the number of P-cadherin-positive cells in interfollicular epidermis (IFE; data are mean +- SD; n = 3 mice per group; ****, P < 0.0001; Student's t test) and MC number colocalizing with P-cadherin-positive cell clusters in hair follicles (data are pooled and depicted as mean +- SD; n = 5 mice per group; **, P = 0.0095; unequal variances Student's t test with Welch's correction). (F) Quantitative RT-PCR for P-cadherin RNA expression in control and Par3 KO primary KCs cultured at LC or 5 d HC. Data are pooled and represented as mean +- SEM. n = 5 biological replicates per group. LC: ns, P = 0.0940. HC: ns, P = 0.941. One-way ANOVA with Tukey posthoc test was used. (G) Western blot analysis

P-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunohistochemistry (Frozen)

P-cadherin Antibody in Immunohistochemistry (IHC) — Figure 4. Depletion of Abi1 affects hair follicle development and distribution of the hair-follicle transcription factor SOX9. (A) Whole-mount immunofluorescence of control and Abi1-2082 KD E16.5 embryo immunostained for the hair follicle marker P-cadherin. (B and C) Quantification of data shown in A. Data are the means +- SD from four embryos per condition. *, P = 5 x 10 -3 (germs); *, P = 7 x 10 -5 (pegs) for control versus Abi1-2082 KD cells by unpaired t test. (D-F) Sagittal views of 10-um sections of dorsal skin from control and Abi1-2082 KD E16.5 embryos. Sections were immunostained for P-cadherin and SOX9. Arrows indicate abnormal distribution of SOX9. (G) Quantification of basal layer SOX9 staining shown in E and in sections from embryos injected in the same manner with shWasf2-493;H2B-GFP lentivirus. Data are the mean +- SD from four control, four Abi1, and three Wasf2 embryos. *, P = 0.0017 (Ctrl vs. Abi1-2082 ); *, P = 0.024 (Ctrl vs. Wasf2-493 ) by unpaired t test. (H) Whole-mount immunofluorescence of control and Abi1-2082 KD E16.5 embryos immunostained for SOX9. In the images, insets show the transduced cells (H2B - GFP + ), and dotted lines indicate the dermal-epidermal border. Nuclei were stained with DAPI (blue). Scale bars = 100 um (A), 20 um (D and F), and 50 um (E and H). FOV, field of view.

P-cadherin Antibody in Western Blot (WB) — Figure 6. P-cadherin expression is sufficient to induce MC dedifferentiation and hyperproliferation. (A) TRP2 immunostaining of back skin cross sections of P0 control and E-cad eKO mice. Data are representative of three independent experiments. (B) Quantification of MC number in interfollicular epidermis of control and E-cad eKO P0 back skin. Data are pooled and represented as mean +- SD. n = 3 mice per group. *, P = 0.0133; Student's t test. (C) Western blot analysis for P- and E-cadherin expression in control, E-cad KO (KO), and E-cad KO /P-cad KD (KK) KCs. Data are representative of three independent experiments. (D) Phase-contrast micrographs of MCs co-cultured with control (left), E-cad KO (middle), or E-cad KO / P-cad KD (right) KCs for 3 d at HC. Data are representative of three independent experiments. (E) Quantification of D (eight images per condition). Data are pooled and represented as mean +- SD. Dendritic length: n = 3 biological replicates per group; Ctrl versus KO: ***, P = 0.0003; KO versus KK: ***, P = 0.0005; one-way ANOVA with Tukey posthoc test. MC numbers: n = 3 biological replicates per group; Ctrl versus KO: **, P = 0.0038; KO versus KK: **, P = 0.0042; one-way ANOVA with Tukey posthoc test. (F) Western blot analysis for P-cadherin in control- and P-cadherin-expressing CHOs. Data are representative of two independent experiments. (G) Quantification of MC number co-cultured with control or P-cadherin overexpressing CHO cells for 4 d at HC (20 image

Product Details

13-2000Z

Applications	Tested Dilution	Publications
Western Blot (WB)	10 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 3 publications 3 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 6 publications 6 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Inhibition Assays (IA)	60 µg/mL	-
In vitro Assay (IV)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Mouse
Published species	Bovine, Fish, Hamster, Human, Mouse, Rat, Sheep, Tag
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	PCD-1
Immunogen	Mouse endoderm cell line PSA5-E. View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	2.0 mg/mL
Purification	Ion-exchange chromatography
Storage buffer	10mM PBS, pH 7.4, with 1% BSA
Contains	no preservative
Storage conditions	4° C
RRID	AB_2533006

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell-line with spleen cells of Donryu rat after immunization with mouse endoderm cell line PSA5-E.

Specificity: This antibody specifically reacts with mouse P-cadherin. This antibody inhibits P-cadherin-dependent cell-cell contact.

Cross reactivity: This antibody does not react with human or chicken P-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µl or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

Target Information

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin.

Developmental biology

Vonica A,Bhat N,Phan K,Guo J,Iancu L,Weber JA,Karger A,Cain JW,Wang ECE,DeStefano GM,O'Donnell-Luria AH,Christiano AM,Riley B,Butler SJ,Luria V

13-2000Z was used in Immunocytochemistry-immunoflourescence to show that the Bone Morphogenetic Protein (BMP) and Wnt signaling pathways share Apcdd1 as a common inhibitor and that Apcdd1 is a taxon-restricted gene with novel domains and signaling functions.

Sat Aug 01 00:00:00 EDT 2020

Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin.

Developmental biology

Vonica A,Bhat N,Phan K,Guo J,Iancu L,Weber JA,Karger A,Cain JW,Wang ECE,DeStefano GM,O'Donnell-Luria AH,Christiano AM,Riley B,Butler SJ,Luria V

Sat Aug 01 00:00:00 EDT 2020

Skin vasculature and hair follicle cross-talking associated with stem cell activation and tissue homeostasis.

eLife

Li KN,Jain P,He CH,Eun FC,Kang S,Tumbar T

13-2000Z was used in Immunohistochemistry-immunofluorescence to suggest a working model in which coordinated remodeling and molecular cross-talking of the adult epithelial and endothelial skin compartments modulate timing of HFSC activation from quiescence for proper tissue homeostasis of adult skin.

Thu Jul 25 00:00:00 EDT 2019

The Wave complex controls epidermal morphogenesis and proliferation by suppressing Wnt-Sox9 signaling.

The Journal of cell biology

Cohen J,Raviv S,Adir O,Padmanabhan K,Soffer A,Luxenburg C

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunohistochemistry

Mon Apr 01 00:00:00 EDT 2019

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Western Blot

Wed Feb 01 00:00:00 EST 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Western Blot

Wed Feb 01 00:00:00 EST 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

The Journal of experimental medicine

Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Western Blot

Wed Feb 01 00:00:00 EST 2017

Retinal pigment epithelial integrity is compromised in the developing albino mouse retina.

The Journal of comparative neurology

Iwai-Takekoshi L,Ramos A,Schaler A,Weinreb S,Blazeski R,Mason C

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Thu Dec 15 00:00:00 EST 2016

Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth.

Development (Cambridge, England)

Tomann P,Paus R,Millar SE,Scheidereit C,Schmidt-Ullrich R

132000Z was used in immunohistochemistry - paraffin section to identify nuclear factor-kappa B targeted genes involved in hair follicle development

Sun May 01 00:00:00 EDT 2016

CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells.

Journal of cell science

Grudzien-Nogalska E,Reed BC,Rhoads RE

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Thu May 15 00:00:00 EDT 2014

Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration.

Development (Cambridge, England)

Veniaminova NA,Vagnozzi AN,Kopinke D,Do TT,Murtaugh LC,Maillard I,Dlugosz AA,Reiter JF,Wong SY

Sun Dec 01 00:00:00 EST 2013

BMI1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor.

Nature cell biology

Biehs B,Hu JK,Strauli NB,Sangiorgi E,Jung H,Heber RP,Ho S,Goodwin AF,Dasen JS,Capecchi MR,Klein OD

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2013

BMI1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor.

Nature cell biology

Biehs B,Hu JK,Strauli NB,Sangiorgi E,Jung H,Heber RP,Ho S,Goodwin AF,Dasen JS,Capecchi MR,Klein OD

Published figure using P-cadherin monoclonal antibody (Product # 13-2000Z) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2013

Abnormal placental development and early embryonic lethality in EpCAM-null mice.

PloS one

Nagao K,Zhu J,Heneghan MB,Hanson JC,Morasso MI,Tessarollo L,Mackem S,Udey MC

13-2000Z was used in immunohistochemistry - frozen section to review data regarding EpCAM

Thu Dec 31 00:00:00 EST 2009

P-cadherin is a p63 target gene with a crucial role in the developing human limb bud and hair follicle.

Development (Cambridge, England)

Shimomura Y,Wajid M,Shapiro L,Christiano AM

13-2000Z was used in immunohistochemistry - frozen section to investigate if p63 directly interacts with two distinct regions of the P-cadherin gene CDH3 promoter

Fri Feb 01 00:00:00 EST 2008

Tissue surface tensions guide in vitro self-assembly of rodent pancreatic islet cells.

Developmental dynamics : an official publication of the American Association of Anatomists

Jia D,Dajusta D,Foty RA

13-2000Z was used in flow cytometry to investigate how differential adhesion alters the organization of immortalized rodent pancreatic islet cells

Wed Aug 01 00:00:00 EDT 2007

Cadherin expression in the developing mouse olfactory system.

The Journal of comparative neurology

Akins MR,Benson DL,Greer CA

13-2000Z was used in immunohistochemistry to report the localization of classical cadherins in the developing olfactory system.

Sun Apr 01 00:00:00 EDT 2007

The chromatin remodeler Mi-2beta is required for establishment of the basal epidermis and normal differentiation of its progeny.

Development (Cambridge, England)

Kashiwagi M,Morgan BA,Georgopoulos K

13-2000Z was used in immunohistochemistry to determine the role of Mi-2beta in skin development

Sun Apr 01 00:00:00 EDT 2007

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.

American journal of physiology. Renal physiology

Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR

Mon Aug 01 00:00:00 EDT 2005

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 EST 2004

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 EST 2004

Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.

The Journal of clinical investigation

Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS

Tue Jul 01 00:00:00 EDT 2003

Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.

The Journal of clinical investigation

Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS

Tue Jul 01 00:00:00 EDT 2003

Links between signal transduction, transcription and adhesion in epithelial bud development.

Nature

Jamora C,DasGupta R,Kocieniewski P,Fuchs E

Thu Mar 20 00:00:00 EST 2003

Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.

Developmental biology

Duguay D,Foty RA,Steinberg MS

13-2000Z was used in flow cytometry to examine cadherin-subtype-specific binding specificities.

Wed Jan 15 00:00:00 EST 2003

Genetic dissection of cadherin function during nephrogenesis.

Molecular and cellular biology

Dahl U,Sjödin A,Larue L,Radice GL,Cajander S,Takeichi M,Kemler R,Semb H

13-2000Z was used in immunohistochemistry to determine if R-cadherin is required for kidney ontogeny.

Fri Mar 01 00:00:00 EST 2002

Ectopic expression of PA2.26 antigen in epidermal keratinocytes leads to destabilization of adherens junctions and malignant progression.

Laboratory investigation; a journal of technical methods and pathology

Scholl FG,Gamallo C,Quintanilla M

13-2000Z was used in western blot to report that expression of the PA2.26 antigen by premalignant keratinocytes induces a fully transformed and metastatic phenotype.