|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 55 and 198 of Human P27 Kip1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-27188 targets P27 Kip1 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-27188 immunogen is recombinant fragment corresponding to a region within amino acids 55 and 198 of Human P27 Kip1.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
IP-MS enrichment of p27 Kip1 (LFQ intensity): p27 Kip1 was enriched 11898-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and p27 Kip1 antibody (Part No. PA5-27188). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo.
PA5-27188 was used in immunohistochemistry to determine tissue specific functions of DLL1 or DLL4 using transgenic mice.
|Preuße K,Tveriakhina L,Schuster-Gossler K,Gaspar C,Rosa AI,Henrique D,Gossler A,Stauber M||PLoS genetics (11:null)||2015|