Western blot analysis of P2X4 / P2X4R in Human Kidney (A), Human Liver (B) and Rat Kidney (C) lysate (35µg protein in RIPA buffer). Samples were probed with the P2X4 / P2X4R antibody (Product # PA5-18994, 1µg / ml ) for 1 hour. Western blot was detected by chemiluminescence.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (YREKKYKYVEDYEQ) corresponding to the C-terminus amino acids of P2RX4|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine and canine based on sequence homology.
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.