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Search Thermo Fisher Scientific
PA1-926 detects rat sodium/calcium-potassium exchanger (NCKX2) overexpressed in HEK cells transfected with the rat gene.
PA1-926 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 78 kDa protein representing recombinant rat NCKX2. Immunohistochemical staining of rat NCKX2 in HEK cells transfected with the rat gene with PA1-926 results in staining of the plasma membrane.
PA1-926 immunizing peptide corresponds to amino acid residues 90-102 from rat NCKX2. This sequence is predicted to be on the extracellular side of the plasma membrane. PA1-926 immunizing peptide (Cat. # PEP-031) is available for use in neutralization and control experiments.
NCKX2, also designated solute carrier family 24, member 2 (SLC24A2), belongs to a family of potassium-dependent sodium/calcium exchangers, all of which contain two large hydrophilic loops and two sets of multiple transmembrane-spanning segments. NCKX2 is expressed in neurons throughout the brain, with high expression found in cone photoreceptors in the retina as well as hippocampal pyramidal cells and the deep nuclei of the cerebellum. A critical component in the process of light adaptation, NCKX2 transports one calcium ion and one potassium ion in exchange for four sodium ions, thereby contolling calcium concentration in the eye during light and darkness. Additionally, NCKX2 regulates calcium levels in other cellular locations that experience rapid calcium fluxes, such as neuronal synapses. Inactivation of NCKX2 is believed to be an important regulatory mechanism in the control of sodium and calcium levels under different physiological conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Na(+)/K(+)/Ca(2+)-exchange protein 2; potassium-dependent sodium-calcium exchanger; Retinal cone Na-Ca+K exchanger; Sodium/potassium/calcium exchanger 2; Solute carrier family 24 member 2; solute carrier family 24, member 2
Gene Aliases: Nckx2; Slc24a2
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