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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the C-term of human PA28-alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman"e;s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Spectroimmunohistochemistry: a novel form of MALDI mass spectrometry imaging coupled to immunohistochemistry for tracking antibodies.
38-2400 was used in immunohistochemistry to evaluate a new MALDI mass spectrometry technique for analyzing antibody staining
|Longuespée R,Boyon C,Desmons A,Kerdraon O,Leblanc E,Farré I,Vinatier D,Day R,Fournier I,Salzet M||Omics : a journal of integrative biology (18:132)||2014|
11S regulator complex alpha subunit; 11S regulator complex subunit alpha; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; IFI5111; IGUP I-5111; interferon gamma up-regulated I-5111 protein; interferon-; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; PA28 alpha subunit; PA28A; PA28alpha; protease (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); proteasome activator 28 subunit alpha; proteasome activator complex subunit 1; REG-alpha; REGalpha
AW413925; HEL-S-129m; IFI5111; PA28A; PA28alpha; PSME1; REGalpha