Invitrogen

PTPRCAP Polyclonal Antibody

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PTPRCAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human PTPRCAP in human lymph node with PTPRCAP Polyclonal Antibody (Product # PA5-118224, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

PTPRCAP Antibody in Western Blot (WB) — Western Blot using PTPRCAP Polyclonal Antibody (Product # PA5-118224) at 1:500 dilution. Lane A: HL60 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 34 kDa.

PTPRCAP Antibody in Immunoprecipitation (IP) — PTPRCAP Immunoprecipitation using: Lane A: 0.5 mg HL60 Whole Cell Lysate 4 µL with PTPRCAP Polyclonal Antibody (Product # PA5-118224) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PTPRCAP Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 34 kDa.

Product Details

PA5-118224

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:2,000	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human PTPRCAP fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902824

Target Information

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1 can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD45 associated protein; CD45-AP; CD45-associated protein; Lymphocyte phosphatase-associated phosphoprotein; Protein tyrosine phosphatase receptor type C-associated protein; protein tyrosine phosphatase, receptor type, c polypeptide-associated protein; protein tyrosine phosphatase, receptor type, C-associated protein; PTPRC-associated protein

Gene Aliases: CD45-AP; LPAP; PTPRCAP

UniProt ID: (Human) Q14761

Entrez Gene ID: (Human) 5790

Function(s)

defense response