Despite its predicted molecular weight, PIG-Y often migrates at 28-30kDa in SDS-PAGE. A suggested positive control is human spleen tissue lysate.
PA5-20652 can be used with blocking peptide PEP-0772.
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
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Protein Aliases: Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-Y; PREY; protein preY
Gene Aliases: 1810008A14Rik; HPMRS6; PIG-Y; PIGY; Pigyl