In ICC/IF, autophagosome formation has been seen in HeLa cells after treatment with 50uM chloroquine.
Suggested positive control: human brain lysate, LC3 overexpression lysate.
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; RP11-346K17.1
Gene Aliases: 1010001H21Rik; 4922501H04Rik; ATG8E; BOS_13394; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1LC3A; zgc:77094