PA5-27692 targets Cbl-b in WB applications and shows reactivity with Human samples.
The PA5-27692 immunogen is recombinant fragment corresponding to a region within amino acids 735 and 980 of Human Cbl-b.
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E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.
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Protein Aliases: Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene B, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cbl-b; E3 ubiquitin-protein ligase CBL-B; RING finger protein 56; RING-type E3 ubiquitin transferase CBL-B; RNF56; SH3-binding protein CBL-B; Signal transduction protein CBL-B
Gene Aliases: Cbl-b; CBLB; Nbla00127; RNF56
UniProt ID: (Human) Q13191
Entrez Gene ID: (Human) 868
Molecular Function: ligase