Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung tissue.
This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. This gene may play a role in the development of tumors of the kidney and larynx. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.
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Protein Aliases: FLJ40908; FLJ44184; fork head-related protein like A; Fork head-related protein-like A; Forkhead box protein P4; OTTHUMP00000043536; winged-helix repressor FOXP4
Gene Aliases: FKHLA; FOXP4; hFKHLA
UniProt ID: (Human) Q8IVH2
Entrez Gene ID: (Human) 116113