Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is also detectable in human cancers including those of the ovary, lung, and testis.
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Protein Aliases: brain; facilitated glucose transporter member 3; glucose transporter type 3; Glucose transporter type 3, brain; GLUT-3; GLUT3; solute carrier family 2; solute carrier family 2 (facilitated glucose transporter), member 3; Solute carrier family 2 A3 (neuron glucose transporter); Solute carrier family 2, facilitated glucose transporter member 3; solute carrier family 2, member 2; solute carrier family 2, member 3
Gene Aliases: Glut-3; GLUT3; SLC2A3