Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is anterior pituitary tissue.
TSH is a pituitary hormone of 28kDa, which stimulates thyroid growth and production of thyroid hormones. This antibody labels thyrotropic cells of the pituitary.
Protein Aliases: thyroid stimulating hormone, beta; Thyroid-stimulating hormone subunit beta; Thyrotropin alfa; Thyrotropin beta chain; Thyrotropin subunit beta; TSH; TSH-B
Gene Aliases: TSH-B; TSH-BETA; TSHB
UniProt ID: (Human) P01222
Entrez Gene ID: (Human) 7252