Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is thyroid or lung carcinoma.
Thyroid transcription factor-1 (TTF-1), also known as NK2 homeobox 1(NKX2-1), is a member of the NKx2 family of homeodomain transcription factors. It binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. TTF-1 is important in the maintenance of thyroid differentiation and lung development. It is expressed in epithelial cells of the thyroid gland and the lung, and tumors derived from such epithelial cells. TTF-1 is usually found in primary lung adenocarcinomas, small cell carcinomas and thyroid cancer. It is absent in mesotheliomas, breast cancer and colon cancer.
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Protein Aliases: RNA polymerase I termination factor; Transcription termination factor 1; Transcription termination factor I; transcription termination factor, RNA polymerase I; TTF-1; TTF-I
Gene Aliases: TTF-1; TTF-I; TTF1
UniProt ID: (Human) Q15361
Entrez Gene ID: (Human) 7270