Recommended positive controls: A431, H1299, HeLa, HepG2.
Predicted reactivity: Rat (80%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Anti-human Polynucleotide Kinase Mouse Monoclonal Antibody recognizes polynucleotide kinase (PNK), a 57 kDa nuclear enzyme implicated in DNA strand repair processes that occur during DNA replication and recombination, or in response to DNA damaging agents. PNK targets the DNA 5'-hydroxy and 3'-phosphate termini for phosphorylation and dephosphorylation events. During strand breakage in human cells, normal PNK associates with other nuclear proteins to prepare the DNA termini for the enzymatic actions of DNA polymerase-beta and DNA ligase III. PNK is a key functional component in maintaining the genetic stability of human cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); phosphatase/kinase; PKNP; Polynucleotide 3'-phosphatase; Polynucleotide 5'-hydroxyl-kinase; Polynucleotide kinase-3'-phosphatase
Gene Aliases: AOA4; EIEE10; MCSZ; PNK; PNKP
UniProt ID: (Human) Q96T60
Entrez Gene ID: (Human) 11284