|Flow Cytometry (Flow)||2.5 µg/1x10^6 cells|
|Immunocytochemistry (ICC)||5-15 µg/mL|
|Immunofluorescence (IF)||5-15 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||5-15 µg/mL|
|Western Blot (WB)||1-10 µg/mL|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human Cadherin-13 Glu23-Ala692|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. T-cadherin (for truncated-cadherin, also designated heartcadherin or cadherin-13) expression levels have been shown to be reduced in human breast cancers and carcinoma cells lines. Evidence suggests that.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAD13; cadherin 13, H-cadherin (heart); Cadherin-13; CDH13; CDHH; H-cadherin; H-cadherin (heart); Heart Cadherin; P105; T cad; T Cadherin; T-cad; T-cadherin; Truncated Cadherin
Gene Aliases: CDH13; CDHH; P105
UniProt ID: (Human) A8W477
Entrez Gene ID: (Human) 1012
Molecular Function: cadherin
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