The antibody detects endogenous levels of total GANP / MCM3AP protein.
GANP (germinal center-associated nuclear protein) is up-regulated selectively in germinal centers and carries an MCM-binding domain in the carboxyl-terminal side. It was found that a region (from 414 to 550 aa) in GANP molecule that is slightly similar to the known DNA-primase component p49. The recombinant GANP fragment covering this region synthesizes RNA primers for extension by DNA polymerase I with single-stranded DNA templates in vitro. GANP DNA-primase activity is controlled by phosphorylation at Ser(502) that is induced by CD40-mediated signaling in vitro and in the germinal center B cells stimulated with antigen in vivo. Overexpression of ganp cDNA in Daudi B cells caused the increased DNA synthesis more than the levels of the mock-transfectants. These evidences suggested that the novel DNA-primase GANP is involved in regulation of cell proliferation of antigen-driven B cells in germinal centers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 80 kDa MCM3-associated protein; FLJ44336; FLJ45306; GANP; GC-associated DNA primase; germinal center-associated nuclear protein; Germinal-center associated nuclear protein; germinal-centre associated nuclear protein; KIAA0572; MAP80; MCM3 acetylating protein; MCM3 acetyltransferase; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; MCM3AP; minichromosome maintenance deficient 3-associated protein; nuclear protein GANP
Gene Aliases: GANP; KIAA0572; MAP80; MCM3AP; mKIAA0572; SAC3