Keep as concentrated solution.
Positive Control: Raji, Raji membrane fraction extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B220 antibody; C antibody; c polypeptide antibody; CD45; CD45 antibody; CD45 antigen; CD45 antigen antibody; CD45R antibody; GP180 antibody; HCD45; L-CA; LCA antibody; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen antibody; leukocyte common antigen B; leukocyte-common antigen antibody; LY5 antibody; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; PTPRC antibody; receptor type; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C antibody; T200; T200 antibody; T200 glycoprotein; T200 glycoprotein antibody; T200 leukocyte common antigen; T200 leukocyte common antigen antibody
Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; RT7; T200