|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH conjugated, corresponding to the residues L(506) S K S S S Q I R M S G L P A D N L A T(525) of human PAC1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA3-115 detects PAC1 in human samples.
PA3-115 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~40-60 kDa protein representing PAC1 in human samples. In immunohistochemistry, PAC1 immunoreactivity is predominantly localized to the plasma membrane.
PA3-115 immunogen is a synthetic peptide, KLH conjugated, corresponding to the residues L(506) S K S S S Q I R M S G L P A D N L A T(525) of human VPAC2 receptor.
The pituitary adenylate cyclase-activating peptide receptor (PAC1) is a member of a family of three G-protein coupled receptors which mediate VIP (vasoactive intestinal peptide) and PACAP (pituitary adenlyate cyclase activating peptide) function in a variety of tissues. PAC1 demonstrates a higher affinity for PACAP than VIP. Expression of the VIP and VACAP receptors has been linked to the presence of human tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Immunocytochemical identification of VPAC1, VPAC2, and PAC1 receptors in normal and neoplastic human tissues with subtype-specific antibodies.
PA3-115 was used in immunocytochemistry, immunohistochemistry and western blot to produce anti-VPAC1 receptor, anti-VPAC2 receptor and anti-PAC1 antibodies and investigate their expression in human tissues
|Schulz S,Röcken C,Mawrin C,Weise W,Höllt V,Schulz S||Clinical cancer research : an official journal of the American Association for Cancer Research (10:8235)||2004|