Invitrogen

PAF49 Monoclonal Antibody (GT7212)

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PAF49 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PAF49 in U2OS cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with PAF49 monoclonal antibody (Product # MA5-27813) using a dilution of 1:500, followed by Hoechst.

PAF49 Antibody in Western Blot (WB) — Western blot analysis of PAF49 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with PAF49 monoclonal antibody (Product # MA5-27813) using a dilution of 1:500.

Product Details

MA5-27813

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	GT7212
Immunogen	Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.99 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735299

Product Specific Information

Positive Control: 293T, A549, H1299, HCT116

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A34.5; antis; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; DNA-directed RNA polymerase I subunit G; DNA-directed RNA polymerase I subunit RPA34; RNA polymerase I-associated factor PAF49

Gene Aliases: ASE-1; ASE1; CAST; CD3EAP; PAF49; POLR1G

UniProt ID: (Human) O15446

Entrez Gene ID: (Human) 10849

Molecular Function: DNA-directed RNA polymerase