Invitrogen

PAF49 Monoclonal Antibody (GT7212)

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PAF49 Antibody in Western Blot (WB) — Western blot analysis of PAF49 in 293T cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with PAF49 monoclonal antibody (Product # MA5-27813) using a dilution of 1:500.

PAF49 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of PAF49 in U2OS cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with PAF49 monoclonal antibody (Product # MA5-27813) using a dilution of 1:500, followed by Hoechst.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT7212

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735299

Product Specific Information

Positive Control: 293T, A549, H1299, HCT116

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A34.5; antis; Antisense to ERCC-1 protein; ASE-1; CAST; CD3-epsilon-associated protein; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; DNA-directed RNA polymerase I subunit RPA34; RNA polymerase I-associated factor PAF49

Gene Aliases: ASE-1; ASE1; CAST; CD3EAP; PAF49

UniProt ID: (Human) O15446

Entrez Gene ID: (Human) 10849

Function(s)

transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transmembrane receptor protein tyrosine kinase signaling pathway rRNA transcription positive regulation of gene expression, epigenetic

Process(es)

DNA-directed RNA polymerase activity poly(A) RNA binding