Invitrogen

PAI1 Monoclonal Antibody (1D5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

3 References

View all (24) PAI1 antibodies

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Datasheet

Questions & Answers

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PAI1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Platelets (Lane 1), A549 (Lane 2) and A549 treated with TGF beta (5ng/ml for 8hrs) (Lane 3). The blot was probed with Anti-PAI-1 Monoclonal Antibody (Product # MA5-17171, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to PAI-1 was detected in Platelets and is induced in A549 cells upon TGF beta treatment.

PAI1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SERPINE1 monoclonal antibody (Product # MA5-17171) followed with DAB staining.

PAI1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of NIH/3T3 cells using SERPINE1 monoclonal antibody (Product # MA5-17171) (green) and negative control (red).

PAI1 Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PAI-1 using PAI-1 Monoclonal Antibody (Product # MA5-17171) shows induction in expression of PAI-1 in A549 cells upon TGF beta treatment. {TM}

PAI1 Antibody — Fig. 3 Mice bearing lung tumors exhibit elevated expression levels of p53 target genes in their livers. a WTp53 and p53 KO mice were intraperitoneally injected with CCl 4 or with sterile corn oil as a control (NT). After 48 h, mice livers were extracted, total RNA was isolated from the livers, and mRNA levels of p21 and SERPINE1 were measured by qRT-PCR. The analyzed mRNA levels are represented in box plot. Average (horizontal line), standard deviation (box), and highest and lowest reads (error bars) are shown. Unpaired t -test, * P < 0.05, *** P < 0.001. WTp53 NT n = 8 mice, p53 KO NT n = 5 mice, WTp53 treated n = 8 mice, p53 KO treated n = 4 mice). b WTp53 and p53 KO mice were irradiated (IR, 10 Gy) or left non-treated as a control (NT). After 24 h, mice livers were extracted, total RNA was isolated from mice livers, and mRNA levels of p21 and SERPINE1 were measured by qRT-PCR. The analyzed mRNA levels are represented in box plot. Average (horizontal line), standard deviation (box), and highest and lowest reads (error bars) are shown. (Unpaired t -test, * P < 0.05, ** P < 0.01. WTp53 NT n = 8 mice, p53 KO NT n = 5 mice, WTp53 treated n = 4 mice, p53 KO treated n = 6 mice). c - f WTp53 and p53 KO mice were intravenously injected with D122 cancer cells (10 6 cells) or with PBS alone as a control (NT). After 7 days, lung tumors were generated and mice were sacrificed. n = 4 mice. c Representative lung histological sections stained by H&E of D122 cell-derived tumors. Arrows ind

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:200 - 1:1000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200 - 1:400

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D5

Immunogen

Purified recombinant fragment of human SERPINE1 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2538642

Product Specific Information

MA5-17171 targets SERPINE1 in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17171 immunogen is purified recombinant fragment of human SERPINE1 expressed in E. Coli.

MA5-17171 detects SERPINE1 which has a predicted molecular weight of approximately 45kDa.

Target Information

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; plas; Plasminogen activator inhibitor 1; plasminogen activator inhibitor, type I; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1

Gene Aliases: PAI; PAI-1; PAI1; PLANH1; SERPINE1

UniProt ID: (Human) P05121

Entrez Gene ID: (Human) 5054, (Mouse) 18787

Molecular Function: enzyme modulator protease inhibitor serine protease inhibitor