Invitrogen

PAI1 Monoclonal Antibody (1D5)

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PAI1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using SERPINE1 monoclonal antibody (Product # MA5-17171) followed with DAB staining.

PAI1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Platelets (Lane 1), A549 (Lane 2) and A549 treated with TGF beta (5ng/ml for 8hrs) (Lane 3). The blot was probed with Anti-PAI-1 Monoclonal Antibody (Product # MA5-17171, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to PAI-1 was detected in Platelets and is induced in A549 cells upon TGF beta treatment.

PAI1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of NIH/3T3 cells using SERPINE1 monoclonal antibody (Product # MA5-17171) (green) and negative control (red).

PAI1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PAI-1 using PAI-1 Monoclonal Antibody (Product # MA5-17171) shows induction in expression of PAI-1 in A549 cells upon TGF beta treatment. {TM}

Product Details

MA5-17171

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Flow Cytometry (Flow)	1:200 - 1:400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1D5
Immunogen	Purified recombinant fragment of human SERPINE1 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2538642

Product Specific Information

MA5-17171 targets SERPINE1 in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17171 immunogen is purified recombinant fragment of human SERPINE1 expressed in E. Coli.

MA5-17171 detects SERPINE1 which has a predicted molecular weight of approximately 45kDa.

Target Information

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fat-1 Transgenic Mice With Augmented n3-Polyunsaturated Fatty Acids Are Protected From Liver Injury Caused by Acute-On-Chronic Ethanol Administration.

Frontiers in pharmacology

Warner J,Hardesty J,Song Y,Sun R,Deng Z,Xu R,Yin X,Zhang X,McClain C,Warner D,Kirpich I

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunohistochemistry

Thu Sep 29 00:00:00 EDT 2022

MAPKAP Kinase-2 Drives Expression of Angiogenic Factors by Tumor-Associated Macrophages in a Model of Inflammation-Induced Colon Cancer.

Frontiers in immunology

Suarez-Lopez L,Kong YW,Sriram G,Patterson JC,Rosenberg S,Morandell S,Haigis KM,Yaffe MB

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunohistochemistry

Wed Jun 23 00:00:00 EDT 2021

MAPKAP Kinase-2 Drives Expression of Angiogenic Factors by Tumor-Associated Macrophages in a Model of Inflammation-Induced Colon Cancer.

Frontiers in immunology

Suarez-Lopez L,Kong YW,Sriram G,Patterson JC,Rosenberg S,Morandell S,Haigis KM,Yaffe MB

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunohistochemistry

Wed Jun 23 00:00:00 EDT 2021

Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation.

Nature communications

Ghini F,Rubolino C,Climent M,Simeone I,Marzi MJ,Nicassio F

MA5-17171 was used in Western Blotting to conclude a sophisticated regulatory layer of miRNA and gene expression mediated by specific endogenous targets exists in mammalian cells.

Tue Aug 07 00:00:00 EDT 2018

Various stress stimuli rewire the profile of liver secretome in a p53-dependent manner.

Cell death & disease

Charni-Natan M,Solomon H,Molchadsky A,Jacob-Berger A,Goldfinger N,Rotter V

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunohistochemistry

Tue May 29 00:00:00 EDT 2018

Association of autotaxin and lysophosphatidic acid receptor 3 with aggressiveness of human breast carcinoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Popnikolov NK,Dalwadi BH,Thomas JD,Johannes GJ,Imagawa WT

MA5-17171 was used in western blot to evaluate the LPA-producing enzyme autotaxin and LPAR3 as potential therapeutic targets in breast cancer patients

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; plas; Plasminogen activator inhibitor 1; plasminogen activator inhibitor, type I; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1

Gene Aliases: PAI; PAI-1; PAI1; PLANH1; SERPINE1

UniProt ID: (Human) P05121

Entrez Gene ID: (Human) 5054, (Mouse) 18787

Molecular Function: calmodulin-related protease inhibitor