Immunofluorescence analysis of PARP was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PARP Mouse Monoclonal Antibody (436400) at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing both nuclear and cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Horse, Human, Mouse, Rhesus monkey, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein derived from the C-terminal region of human PARP protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
USP17-mediated deubiquitination and stabilization of HDAC2 in cigarette smoke extract-induced inflammation.
436400 was used in western blot to report that USP17 interacts with HDAC2
|Song H,Tao L,Chen C,Pan L,Hao J,Ni Y,Li D,Li B,Shi G||International journal of clinical and experimental pathology (8:10707)||2015|
The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33.
436400 was used in western blot to investigate how IL-33 regulates IL-13 gene expression
|Ni Y,Tao L,Chen C,Song H,Li Z,Gao Y,Nie J,Piccioni M,Shi G,Li B||International journal of molecular sciences (16:27956)||2015|
TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response.
436400 was used in western blot to study the involvement of TIMELESS in the PARP1-mediated DNA damage response
|Young LM,Marzio A,Perez-Duran P,Reid DA,Meredith DN,Roberti D,Star A,Rothenberg E,Ueberheide B,Pagano M||Cell reports (13:451)||2015|
Deubiquitination and stabilization of IL-33 by USP21.
436400 was used in immunoprecipitation to study the effect of ubiquitination modification in regulating the protein stability and the nuclear function of IL-33
|Tao L,Chen C,Song H,Piccioni M,Shi G,Li B||International journal of clinical and experimental pathology (7:4930)||2014|